What it is: 5-MTHF / B12 MC 2000 is a vegetarian, dual-nutrient methylation support formula developed by DaVinci Labs, delivering 1,000 mcg DFE of Folate as L-Methyltetrahydrofolate Calcium (L-MTHF) — the most bioavailable, enzymatically active form of folate — alongside 1,000 mcg of Vitamin B12 as Methylcobalamin, the neurologically active coenzyme form of B12 that requires no hepatic conversion for immediate utilization. Both nutrients are delivered in their most biologically ready forms, bypassing the enzymatic conversion steps required by conventional folic acid and cyanocobalamin supplementation — steps that are frequently compromised in individuals with MTHFR enzyme polymorphisms, malabsorption, poor liver function, or digestive system challenges. Designed for practitioner-directed use in methylation support, homocysteine management, neurological health, and perinatal nutrition protocols, 5-MTHF / B12 MC 2000 addresses the most clinically critical B-vitamin cofactor pair in the one-carbon metabolic cycle within a single, clean-label vegetarian capsule.
How it works: 5-MTHF / B12 MC 2000 supports health through the tightly coupled methylation cycle in which folate and vitamin B12 function as essential and interdependent cofactors. L-Methyltetrahydrofolate (L-MTHF) is the predominant circulating form of folate in human plasma and the direct methyl donor in the folate cycle — donating its methyl group to homocysteine via methionine synthase to produce methionine, which is subsequently converted to S-adenosylmethionine (SAMe) — the universal methyl donor governing over 200 methylation-dependent reactions including neurotransmitter synthesis, DNA methylation, gene expression regulation, phospholipid biosynthesis, and hormonal metabolism. Unlike standard folic acid, L-MTHF bypasses the rate-limiting methylenetetrahydrofolate reductase (MTHFR) enzyme conversion step entirely, making it the preferred folate form for the estimated 40–60% of the population carrying common MTHFR polymorphisms (C677T, A1298C) that impair folic acid conversion. Methylcobalamin, the neurologically active coenzyme form of B12, serves as the direct cofactor for methionine synthase — the enzyme that couples the folate and methionine cycles — and additionally supports myelin sheath synthesis and maintenance, neurological integrity, and the production of red blood cells. Together, these two active-form nutrients support the complete methylation cycle, help maintain healthy homocysteine levels within normal ranges, promote neurological function and nerve integrity, support DNA repair and healthy cell division, and provide the essential cofactors for optimal brain, immune, and cardiovascular function.
When to use it: 5-MTHF / B12 MC 2000 is appropriate for practitioner-directed use in adult clients requiring active-form folate and B12 support for methylation optimization, homocysteine management, neurological health, and cardiovascular protection. It is particularly well-suited for clients with known or suspected MTHFR polymorphisms, those with folate or B12 insufficiency from dietary, absorption, or metabolic causes, clients in the perinatal period where adequate folate status is critical for neural tube development and healthy fetal brain and spinal cord formation, vegans and vegetarians with elevated B12 deficiency risk, and clients on medications that deplete folate or impair B12 absorption. One capsule daily provides a clinically meaningful active-form methylation cofactor dose appropriate for both maintenance and therapeutic protocols.
Serving Size: 1 Vegetarian Capsule
Servings Per Container: 60
Suggested Use: As a dietary supplement, take 1 capsule daily, or as directed by your healthcare practitioner.
| Amount Per Serving | % Daily Value | |
| Folate (as L-Methyltetrahydrofolate Calcium) | 1,000 mcg DFE | 250% |
| Vitamin B12 (as Methylcobalamin) | 1,000 mcg | 41,667% |
Other Ingredients: Microcrystalline cellulose, hypromellose (capsule), vegetarian leucine.
Caution: If pregnant or nursing, consult your healthcare practitioner before taking this product. Keep out of reach of children. Store in a cool, dry place. Color may vary due to natural ingredient variation.
✓ Vegetarian ✓ Gluten Free ✓ Soy Free ✓ Non-GMO
Key Ingredients
Folate (as L-Methyltetrahydrofolate Calcium) — 1,000 mcg DFE L-Methyltetrahydrofolate Calcium (L-MTHF) is the calcium salt of the predominant circulating, biologically active form of folate in human plasma — the end-product of the multi-step enzymatic folate activation pathway — delivered here in its final, immediately utilizable form without requiring the MTHFR enzyme conversion step that standard folic acid supplementation depends upon. As the direct methyl donor in the folate cycle, L-MTHF donates its methyl group to homocysteine via methylcobalamin-dependent methionine synthase, producing methionine for S-adenosylmethionine (SAMe) synthesis — the universal methyl donor governing over 200 downstream methylation reactions including neurotransmitter synthesis, DNA methylation, epigenetic gene regulation, and phospholipid metabolism. For the estimated 40–60% of the population carrying MTHFR C677T or A1298C polymorphisms that impair folic acid conversion, L-MTHF is the clinically essential folate form, ensuring adequate methylation cycle function and supporting neural tube development, homocysteine management, and cellular DNA integrity independent of enzymatic conversion capacity.
Vitamin B12 (as Methylcobalamin) — 1,000 mcg Methylcobalamin is the neurologically active, methyl-group-bearing coenzyme form of vitamin B12 — representing approximately 80% of circulating plasma B12 in healthy individuals — and the form that directly participates in the folate-methionine methylation cycle without requiring hepatic conversion from cyanocobalamin or hydroxocobalamin. As the obligate cofactor for methionine synthase, methylcobalamin couples the folate and methionine cycles by accepting the methyl group from L-MTHF and transferring it to homocysteine, completing the homocysteine remethylation reaction that maintains healthy homocysteine levels within normal ranges and sustains SAMe production for ongoing methylation activity throughout the body. Beyond its methylation cycle role, methylcobalamin is essential for myelin sheath synthesis and maintenance in the peripheral and central nervous system, supporting optic nerve integrity, sensory and motor nerve conduction velocity, and cognitive function — establishing it as the preferred B12 form for neurological health protocols and for the estimated 10–15% of adults over 60 with clinically significant B12 insufficiency due to declining intrinsic factor production.
